In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach

Table 2

Toxicity and antitoxicity levels of CCC, CFCC, and CAF in D. melanogaster.

CCC (mg/mL)	Survival (%)		CFCC (mg/mL)	Survival (%)		CAF (mM)	Survival (%)
	Simple treatment	Combined treatment		Simple treatment	Combined treatment		Simple treatment	Combined treatment
0	100	100	0	100	100	0	100	100
H2O2	—	46.66	H2O2	—	46.66	H2O2	—	46.66
0.7	100	100	0.7	87.66	83.33	0.004	100	54^Δ
3	100	92	3	88.66	100	0.016	100	55.33^Δ
6	100	85.66	6	96.66	84.66	0.032	100	51^Δ
25	100	74.66	25	87.33	75	0.127	100	54.66^Δ
100	92	65	100	77	45.66^Δ	0.51	100	51.33^Δ

Data are expressed as percentage of survival adults with respect to 300 untreated 72-hour-old larvae from three independent experiments. Combined treatments using standard medium and 0.15 M hydrogen peroxide. Asterisks () indicate significant differences (one tail) with respect to the hydrogen peroxide control group and untreated control group: Chi-square value higher than 5.02 [35]. Delta letter (Δ) means significant differences between the same concentrations used in toxicity and antitoxicity assays comparing within the same treated substance.