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Figure 3: Representative isosurface 3D imaging, performed using the Image-Pro Premier® 3D software of the CLSM images biofilms stained with BacLight® (green = viable bacteria; red = nonviable bacteria). (a) Initial biofilm . (b) NCSD after 48 hours of exposure. (c) SNAD after 48 hours of exposure. (d) NGAD after 48 hours of exposure.