Microtubule Polymerization Functions in Hypersensitive Response and Accumulation of H₂O₂ in Wheat Induced by the Stripe Rust

Figure 1

Fluorescence micrographs of hypersensitive cell death localization in incompatible interaction between wheat and Pst (race CYR23) in DMSO-only (control) and oryzalin treatments. (a) In control, haustorial mother cells formed and mesophyll cells showed HR reaction, 24 hai. (b) More than two haustorial mother cells formed treated with oryzalin, 24 hai. (c) Conspicuous HR in mesophyll cells was observed in control, 48 hai. (d) The apex of the infection hypha formed a haustorial mother cell. HR was induced by HMC and the whole cells started to lose original shape treated with oryzalin, 48 hai. (e) In control, many HR cells were visualized in mesophyll cells, 96 hai. (f) Slight HR-like cells appeared in the mesophyll cells treated with oryzalin treatment, 96 hai. GT: germ tube; HMC: haustorial mother cell; HR: hypersensitive response; IH: infection hypha; SP: spore; and SV: substomatal vesicle. Scale bars = 50 μm.

(a)

(b)

(c)

(d)

(e)

(f)