17β-Estradiol Promotes Schwann Cell Proliferation and Differentiation, Accelerating Early Remyelination in a Mouse Peripheral Nerve Injury Model

Figure 1

E2 treatment accelerated early expression of MBP in the nerve bridge upon sciatic nerve transection. (a) A schematic drawing showing the experimental setting. The mice were injected with E2 or solvent 5 days prior to nerve transection (−D5～−D1), and two injections per week were continued for a sustained drug delivery till the mice were sacrificed. The surgery was carried out at D0, and the mice were sacrificed for sample collection at D10, D12, D15, and D30, respectively, after the surgery. (b) Immunofluorescence images of expression of MBP (green signals, white arrow) in the longitudinal section of the nerve bridge (NB) from 10, 12, 15, and 30 day after injury are shown individually. NF staining (red signals) was used to illustrate axons, and some proximal stump (PS) where the MBP signal was regular and strong was retained left of the nerve to distinguish the site of the NB, which is adjacent. Scale bars represent 250 M (100x). MBP, myelin basic protein; NF, neurofilament; PS, proximal stump. (c) Quantification of axonal signal SMI-31R (; NS, no significance).

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