17β-Estradiol Promotes Schwann Cell Proliferation and Differentiation, Accelerating Early Remyelination in a Mouse Peripheral Nerve Injury Model

Figure 4

E2 treatment promoted Schwann cell proliferation. (a) BrdU staining (red signals) for primary Schwann cells treated with E2, E2+MK2206, or DMSO for only 24 h. P75 positive staining (green signals) was used to illustrate immature Schwann cells. Scale bars represent 50 M (400x). (b) BrdU-positive Schwann cell quantification from (a) (, , E2 versus DMSO). (c) BrdU staining (red signals) in the longitudinal section of the nerve bridge site in the injured sciatic nerve from E2-treated and control mice. The c-Jun was costained (green signals) to illustrate immature Schwann cells. Scale bars represent 100 M (200x). (d) BrdU-positive Schwann cell quantification from (c) (, ). BrdU, 5-bromo-2′-deoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole; DMSO, dimethyl sulfoxide.

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