17β-Estradiol Promotes Schwann Cell Proliferation and Differentiation, Accelerating Early Remyelination in a Mouse Peripheral Nerve Injury Model

Figure 5

E2 promotes Schwann cell differentiation and myelination in vitro. (a) MBP staining (green signals) of myelinated DRG explant culture treated with E2, E2+MK2206, or DMSO for 1 week to detect myelin segments, and NF staining (red signals) was used to illustrate axons. Scale bars represent 100 M (200x). (b) Western blot analysis of MBP expression in the DRG explant culture. (c) Quantification of the relative MBP expression intensity based on the results of (b) (, , E2 versus DMSO). (d, e) Relative Sox10 (, , E2 48 h versus DMSO 48 h; NS, E2 12 h versus DMSO 12 h) (d) and Krox20 (, , E2 48 h versus DMSO 48 h; NS, E2 12 h versus DMSO 12 h) (e) mRNA expressions in primary SCs treated with E2, E2+MK2206, or DMSO for 24 h and 48 h, measured using qPCR. (f, g) Relative Mag (, , E2 versus DMSO) (f) and Mpz (, , E2 versus DMSO) (g) mRNA expression in primary SCs treated with E2, E2+MK2206, or DMSO for 48 h, measured using qPCR. DMSO, dimethyl sulfoxide; DRG, dorsal root ganglion; MBP, myelin basic protein; qPCR, quantitative polymerase chain reaction. .

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