BioMed Research International / 2016 / Article / Fig 4

Research Article

Physicochemical Characteristics of Transferon Batches

Figure 4

Electrophoretic profile of Transferon. (a) Method selectivity. The batch 14E14 of Transferon (85 µg) showed 2 main bands at approximately 10 kDa (line 2). A second 14E14 sample (85 µg) exhibited the same electrophoretic pattern when spiked with 5 µg of equine myoglobin (17 kDa) (line 3). A total of 5 µg of equine myoglobin was used as selectivity control (line 4). (b) Batch reproducibility. The electrophoretic patterns of batches 15A01 (line 2) and 15A02 (line 3) of Transferon (100 µg) are consistent. Figure S3 shows the electrophoretic pattern of the 10 analyzed Transferon batches. Electrophoresis was performed in 16% acrylamide gels using a Tris-Gly system. Bands were detected by silver staining.

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