Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression of Pseudomonas aeruginosa In Vitro

Figure 3

The P. aeruginosa glycocalyx was visualized by staining with 50 μg/mL of Alexa Fluor 647 conjugate of Con A. Static biofilm assays under atmospheric pressure (AP) and negative pressure (NP) were shown. P. aeruginosa (green) under negative pressure conditions (d–f) were apt to be small aggregates and exhibited a reduced capacity for biofilm (red) adherence on the cover glass relative to the control (a–c) at 24 h.

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