Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis

Figure 2

Results of COE-PCR for the 8th primer set. Both primary PCR reactions (lanes 1 and 2) produced single bands estimated at 450 bp and 2250 bp (indicated by white arrows), which matched the expected size. Since single bands were obtained, gel purification was unnecessary. The secondary PCR reaction (lane 3) produced a single band estimated at 2.7 kb (indicated by black arrow), which matched the expected size.