Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis

Table 3

Results of primary and secondary PCR reactions with different primer sets.

Primer set	First primary PCR			Second primary PCR			Secondary PCR
	Estimated band size	Optimization required?	Gel excision required?	Estimated band size	Optimization required?	Gel excision required?	Estimated band size
1	400	No	No	1500	No	Yes	1900
2	500	Yes	Yes	1400	No	Yes	1900
3	900	No	No	1000	No	No	1900
4	1000	No	No	900	No	No	1900
5	1100	No	No	800	No	Yes	1900
6	1400	Yes	Yes	500	No	No	1900
7	1600	No	No	300	No	No	1900
8	450	No	No	2250	No	No	2700