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BioMed Research International
Volume 2016, Article ID 8091353, 9 pages
http://dx.doi.org/10.1155/2016/8091353
Research Article

The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b+Gr1+ Cells in a Transgenic Mouse Model

1Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), 07360 Mexico City, CDMX, Mexico
2Unit of Health Sciences, Faculty of Odontology, Universidad Autónoma de Baja California, 21100 Mexicali, BC, Mexico
3Research Unit, Hospital Juárez de México, 07760 Mexico City, CDMX, Mexico
4McArdle Laboratory for Cancer Research, University of Wisconsin, School of Medicine and Public Health, Madison, WI 53726, USA

Received 2 February 2016; Revised 5 May 2016; Accepted 13 June 2016

Academic Editor: Kevin M. Coombs

Copyright © 2016 Gabriela Damian-Morales et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The aim of this study was to analyze the effects of the HPV16 E7 oncoprotein on dendritic cells (DCs) and CD11b+Gr1+ cells using the K14E7 transgenic mouse model. Materials and Methods. The morphology of DCs was analyzed in male mouse skin on epidermal sheets using immunofluorescence and confocal microscopy. Flow cytometry was used to determine the percentages of DCs and CD11b+Gr1+ cells in different tissues and to evaluate the migration of DCs. Results. In the K14E7 mouse model, the morphology of Langerhans cells and the migratory activity of dendritic cells were abnormal. An increase in CD11b+Gr1+ cells was observed in the blood and skin of K14E7 mice, and molecules related to CD11b+Gr1+ chemoattraction (MCP1 and S100A9) were upregulated. Conclusions. These data suggest that the HPV16 E7 oncoprotein impairs the function and morphology of DCs and induces the systemic accumulation of CD11b+Gr1+ cells.