The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity of Arcobacter spp. in a Wastewater Treatment Plant

Table 1

Number of isolates and strains of the identified Arcobacter species found from wastewater using the two molecular methods (m-PCRand 16S rRNA-RFLP) in parallel.

Species	Number of isolates (%)	Number of strains (%)	% genetic diversity	Molecular identification
				/16S
A. butzleri (Ab)	354 (54.4)	247 (58.3)	69.8%	Ab/Ab
A. cryaerophilus (Ac)	226 (34.7)	150 (35.4)	66.4%	Ac/Ac
A. thereius (At)	37 (5.7)	9 (2.1)	24.3%	Ac/Ab
A. skirrowii (As)	16 (2.5)	5 (1.2)	31.3%	As/As
A. (Ad)	12 (1.8)	8 (1.9)	66.7%	~230 bp/Ad
A. ellisii (Ae)	3 (0.5)	2 (0.5)	66.7%	Ac/Ae
A. (Aclo)	2 (0.3)	2 (0.5)	100%	Ac/Aclo
A. nitrofigilis (An)	1 (0.2)	1 (0.2)	100%	As/An
Total	651	424	65.1%

The isolates were genotyped with ERIC-PCR to determine the ones that showed the same ERIC-pattern and therefore belonged to the same strain. et al. [19] and et al. [20]. Results of the RFLP were verified by sequencing the rpoB gene. species recognized on the basis of the new RFLP pattern described by Figueras et al [20]. species recognized on the basis of the new RFLP pattern and described by Levican et al. [21].