Genomic Analysis Unravels Reduced Inorganic Sulfur Compound Oxidation of Heterotrophic Acidophilic Acidicaldus sp. Strain DX-1

Figure 2

RT-qPCR results depicting the expression difference of targeted genes involved in RISC oxidation in Acidicaldus sp. strain DX-1 (in organic matter supplemented with sulfur (S⁰) medium or without sulfur). These genes used in this experiment (the encoding proteins and genomic loci are shown in parentheses) include hdrA (pyridine nucleotidedisulfide oxidoreductase; scaffold254: 1-941), hdrB (heterodisulfide reductase subunit B, homolog; scaffold254: 827-2329), hdrC (iron-sulfur cluster-binding protein; scaffold254: 2500-3246), hdrB2 (heterodisulfide reductase subunit B, homolog; scaffold9: 22130-22789), rhd (rhodanese, thiosulfate sulfurtransferase; scaffold153: 15026-15202), doxD (thiosulfate: quinone oxidoreductase; scaffold68: 10659-11696), sat (sulfate adenylyltransferase; scaffold217: 14172-14891), sqr (sulfide quinone reductase; scaffold18: 14834-16117), and sqr2 (sulfide quinone reductase; scaffold92: 4297-5430).