|
| Methods | Molecular weight | Origin | Authors |
|
Hot water at 80°C and 120°C
0,5% Na2CO3 aqueous solution at 80°C and 120°C | 20–400 kDa
<100 kDa | Cocoon | Gimenes et al. [69] |
LiSCN saturated solution
Silk gland fluid, shaken gently for 30 min | 20–400 kDa | Cocoon
Silk gland | Takasu et al. [41] |
Autoclave at 120°C for 30 min
Heated at 100°C for 10 min | 12–66 kDa | Cocoon | Yang et al. [49] |
| 0,2% Na2CO3 solution heated at 95°C for 120 min | 6 and 67 kDa | Cocoon | Terada et al. [65] |
Autoclave at 120°C for 30 min
Heated at 95°C for 120 min | 10–70 kDa | Cocoon | Terada et al. [70] |
| Distilled water and bromelain solution heated at 55°C for 60 min | 10–250 kDa | Cocoon | Sonjui et al. [71] |
Autoclave at 120°C for 60 min
1,25% citric acid solution heated for 30 min
0,2% Na2CO3 solution
8 M urea solution | 20–220 kDa | Cocoon | Aramwit et al. [51] |
| Autoclave at 100°C and 105°C for 60 min | — | Cocoon | Kodama [48] |
| Autoclave | — | Cocoon | Li et al. [66]; Keawkorn et al. [68]; Li et al. [72]; Kaewkorn et al. [73]; Aramwit et al. [74]; Kitisin et al. [75] |
| Soaking, water decoction, filtration and condensation. | — | Cocoon | Song et al. [76] |
| Heated in deionized water at 95°C for 120 min | — | Cocoon | Kato et al. [10]; Sasaki et al. [61];
Sasaki et al. [77]; Zhaorigetu et al. [78]; Okazaki et al. [79] |
| Autoclave for 30 min, followed by enzymatic hydrolysis by protease | — | Cocoon | Onsa-ard et al. [55] |
| 110°C for 300 min | 65 kDa | Cocoon | Kim et al. [64] |
| 1% NaCl solution | — | Cocoon | Ali and Arumugam Sarasa [80] |
| 0,2% Na2CO3 solution heated at 95°C for 120 min | — | Cocoon | Zhaorigetu et al. [62]; Okazaki et al. [63]; Sasaki et al. [81]; Zhaorigetu et al. [82]; Ogawa et al. [83] |
| Autoclave at 121°C for 60 min | 50–150 kDa | Cocoon | Aramwit et al. [39] |
| Autoclave at 120°C for 60 min | — | Cocoon | Aramwit et al. [9]; Chlapanidas et al. [84]; Saetae and Magaraphan [85]; Khampieng et al. [86]; Aramwit et al. [87]; Purwar et al. [88]; Siritientong and Aramwit [89] |
| Fluid at the anterior and the middle silk gland | 20–400 kDa | Silk gland | Sprague [90] |
| Autoclave at 120°C for 40 min | 20–400 kDa | Cocoon | Da Silva et al. [59] |
| Autoclave at 110°C for 480 min | — | Cocoon | Lee et al. [91] |
| 0,02 M Na2CO3 solution heated for 45 or 60 min | — | Cocoon | Martínez-Mora et al. [92]; Panilaitis et al. [93] |
| Electrolytic alkaline water at 95°C for 7 or 13 hours | 5–18 kDa | Cocoon | Ogino et al. [94] |
| Autoclave at 120°C for 60 min | 200 kDa | Cocoon | Turbiani et al. [95] |
| Autoclave | 14 and 97 kDa | Cocoon | Wu et al. [11] |
| Autoclave at 120°C for 60 min | 10–200 kDa | Cocoon | Zhang et al. [96] |
| Autoclave at 120°C for 40 min | — | Cocoon | Da Silva el al. [97] |
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