Table 2: Diverse methods of extraction of sericin of B. mori, molecular weight and its origin.

MethodsMolecular weightOriginAuthors

Hot water at 80°C and 120°C
0,5% Na2CO3 aqueous solution at 80°C and 120°C
20–400 kDa
<100 kDa
CocoonGimenes et al. [69]
LiSCN saturated solution
Silk gland fluid, shaken gently for 30 min
20–400 kDaCocoon
Silk gland
Takasu et al. [41]
Autoclave at 120°C for 30 min
Heated at 100°C for 10 min
12–66 kDaCocoonYang et al. [49]
0,2% Na2CO3 solution heated at 95°C for 120 min6 and 67 kDaCocoonTerada et al. [65]
Autoclave at 120°C for 30 min
Heated at 95°C for 120 min
10–70 kDaCocoonTerada et al. [70]
Distilled water and bromelain solution heated at 55°C for 60 min10–250 kDaCocoonSonjui et al. [71]
Autoclave at 120°C for 60 min
1,25% citric acid solution heated for 30 min
0,2% Na2CO3 solution
8 M urea solution
20–220 kDaCocoonAramwit et al. [51]
Autoclave at 100°C and 105°C for 60 minCocoonKodama [48]
AutoclaveCocoonLi et al. [66]; Keawkorn et al. [68]; Li et al. [72]; Kaewkorn et al. [73]; Aramwit et al. [74]; Kitisin et al. [75]
Soaking, water decoction, filtration and condensation.CocoonSong et al. [76]
Heated in deionized water at 95°C for 120 minCocoonKato et al. [10]; Sasaki et al. [61];
Sasaki et al. [77]; Zhaorigetu et al. [78]; Okazaki et al. [79]
Autoclave for 30 min, followed by enzymatic hydrolysis by proteaseCocoonOnsa-ard et al. [55]
110°C for 300 min65 kDaCocoonKim et al. [64]
1% NaCl solutionCocoonAli and Arumugam Sarasa [80]
0,2% Na2CO3 solution heated at 95°C for 120 minCocoonZhaorigetu et al. [62]; Okazaki et al. [63]; Sasaki et al. [81]; Zhaorigetu et al. [82]; Ogawa et al. [83]
Autoclave at 121°C for 60 min50–150 kDaCocoonAramwit et al. [39]
Autoclave at 120°C for 60 minCocoonAramwit et al. [9]; Chlapanidas et al. [84]; Saetae and Magaraphan [85]; Khampieng et al. [86]; Aramwit et al. [87]; Purwar et al. [88]; Siritientong and Aramwit [89]
Fluid at the anterior and the middle silk gland20–400 kDaSilk glandSprague [90]
Autoclave at 120°C for 40 min20–400 kDaCocoonDa Silva et al. [59]
Autoclave at 110°C for 480 minCocoonLee et al. [91]
0,02 M Na2CO3 solution heated for 45 or 60 minCocoonMartínez-Mora et al. [92]; Panilaitis et al. [93]
Electrolytic alkaline water at 95°C for 7 or 13 hours5–18 kDaCocoonOgino et al. [94]
Autoclave at 120°C for 60 min200 kDaCocoonTurbiani et al. [95]
Autoclave14 and 97 kDaCocoonWu et al. [11]
Autoclave at 120°C for 60 min10–200 kDaCocoonZhang et al. [96]
Autoclave at 120°C for 40 minCocoonDa Silva el al. [97]