Research Article
The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca2+]i
Figure 3
Effect of metabotropic glutamate receptor agonists DHPG and ACPD on retinal astrocytes in situ. (a) Representative trace showing fura-2 fluorescence ratio of an astrocyte treated twice with 50 μM DHPG followed by 10 μM ATP. The astrocyte responded to ATP, but not DHPG. (b) The mean response elicited by DHPG was not significantly different from baseline, but the mean response elicited by ATP was. (c) Representative trace showing fura-2 fluorescence ratio of an astrocyte treated twice with 50 μM ACPD followed by 10 μM ATP. The astrocyte responded to ATP, but not ACPD. (d) Mean response elicited by ACPD application was not significantly different from baseline, while mean response elicited by ATP was. Error bars are SEM, = number of retinas studied, ns: not significant; at α = 0.05 as determined by Dunn’s multiple comparison test following Kruskal-Wallis ANOVA.
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