Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo

Figure 1

Characteristics and short-term culture of MSCs. (a) Cell morphology was observed under phase-contrast microscopy ((A) magnification: 100x) and differentiation potential was evaluated by Von Kossa, oil red O, and safranin O staining ((B) osteogenesis-magnification: 200x, (C) adipogenesis-magnification: 400x; (D) chondrogenesis-magnification: 200x). (b) The immunophenotype of bone marrow-derived MSCs. Flow cytometry histograms show that cultured MSCs were positive CD29, CD44, CD73, CD90, and CD105. These results show representative histograms of cultured MSCs. (c) Proliferative activity of cultured MSCs. MSCs were cultured on uncoated or poly-L-lysine- (PLL-) coated plates for 5 days. The number of harvested cells was measured by trypan blue staining. The data represent the mean ± standard deviation of three independent experiments (). .

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