Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo

Table 1

Primer sequences.

Gene	Primer sequence (5′-3′)	Annealing temperature (°C)	Product size (bp)
	Forward: CGAATAGTTACGGTCGGAGG; Reverse: GCATGGTTACTGCCTCTGGT	62	309
	Forward: GCGATGGAACTTCGACTTTG; Reverse: CGTTTTCGACCCTGAGAGAGTC	60	285
SH3 (CD73)	Forward: TATTGCACTGGGACATTCGGGT; Reverse: GGTTGCCCATGTTGCATTCTCT	62	443
SH2 (CD105)	Forward: CATCCTTGAAGTCCATGTCCTCTT; Reverse: GCCAGGTGCCATTTTGCTT	62	95
VCAM-1 (CD106)	Forward: GCTTTCCTGCTCCGAAAATCCT; Reverse: AACTGGGCCTTTCGGATGGTAT	62	367
Oct4	Forward: GACAACAATGAGAACCTTCAGGAGA; Reverse: TTCTGGCGCCGGTTACAGAACCA	62	218
Sox2	Forward: AACCAAGACGCTCATGAAGAAG; Reverse: GCGAGTAGGACATGCTGTAGGT	62	341
Nanog	Forward: ATAGCAATGGTGTGACGCAG; Reverse: GATTGTTCCAGGATTGGGTG	62	219
GAPDH	Forward: GTGGTCTCCTCTGACTTCAACA; Reverse: CTCTTCCTCTTGTGCTCTTGCT	62	210