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BioMed Research International
Volume 2016 (2016), Article ID 8208904, 5 pages
http://dx.doi.org/10.1155/2016/8208904
Research Article

Expression of Migration-Related Genes in Human Colorectal Cancer and Activity of a Disintegrin and Metalloproteinase 17

1Department of Internal Medicine, Faculty of Public Health in Bytom, Medical University of Silesia, 40-055 Katowice, Poland
2Department of Molecular Biology, Faculty of Pharmacy in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland

Received 24 October 2015; Accepted 22 February 2016

Academic Editor: Dimitrios Dimitroulis

Copyright © 2016 Katarzyna Walkiewicz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. The ability to form metastases which depends on the mechanisms of cell migration is an important element of the progression of cancer. In the present study we analyzed the genes involved in the regulation of migration in colon cancer cells. Materials and Methods. A total of 20 pairs of surgically removed tumoral and healthy (marginal) tissues samples from colorectal cancer patients at clinical stages I-II and III-IV were analyzed. The isolation of RNA from CRC and normal tissues and its subsequent molecular analysis were performed according to manufacturer’s instructions. Microarray data analysis was performed using the GeneSpring 11.5 platform and Significance Analysis of Microarrays (SAM). In SAM analysis to identify significantly differentially expressed genes score and -value parameters were used. Results. The largest increase in expression of genes was shown by MMP9, ADAM17, EphA2, and TIMP. Conclusions. Presented genes, especially ADAM17, MMP9, EphA2, TIMP1, ICAM 11, and CD4, may be used as prognostic markers of advanced stages of colorectal cancer, contributing to the development of new lines of therapy focused on reducing metastasis of the primary tumor.