SIRT3 Expression Decreases with Reactive Oxygen Species Generation in Rat Cortical Neurons during Early Brain Injury Induced by Experimental Subarachnoid Hemorrhage

Figure 5

Double immunofluorescence straining for expression and cell-specific distribution of SIRT3 protein. Typical photomicrographs illustrated immunofluorescence staining of SIRT3 in cerebral cortex of rats in the sham group and the 24 h post-SAH group. NeuN, GFAP, and DAPI (blue) were used as markers for neurons, astrocytes, and nuclei, respectively. SIRT3 was seldom colocalized with GFAP in the astrocytes in all rats (white circles). By contrast, SIRT3 was abundant and colocalized with NeuN in neurons of sham groups rats (orange color in the merged photomicrographs, indicated by white arrows), which markedly decreased after experimental SAH.