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BioMed Research International
Volume 2016, Article ID 8285428, 27 pages
Research Article

Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots

1Department of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Winchesterstraße 2, 35394 Giessen, Germany
2Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745 Jena, Germany
3Justus-Liebig-University of Giessen, Bioinformatics and System Biology, Heinrich-Buff-Ring 58, 35392 Giessen, Germany
4Justus-Liebig-University of Giessen, Institute for Insect Biotechnology, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany

Received 29 January 2016; Accepted 7 March 2016

Academic Editor: Yudong Cai

Copyright © 2016 Zdeněk Franta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary table 1 shows number of L. sericata transcripts assembled with Trinity and Oases. K-mer sizes vary between 21 and 75 for the Oases assembly. The supplementary figures 1 to 5 show Heat maps with relative expression levels of all L. sericata peptidases. The individual clusters encoding for aspartic (Fig1), cysteine (Fig2), metallo (Fig3), threonine (Fig4) and serine (Fig5) peptidases are depicted on the left, while corresponding clans, families and MEROPS IDs are depicted on the right. Shown are log2-transformed RPKM values (blue resembles lower-expressed genes, while red represents highly expressed genes). The supplementary figure 6 shows the melting curve analysis of all genes which were tested via qRT-PCR.

  1. Supplementary Material