Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing

Figure 3

The seven horizontal bands of points correspond to experiments with mixing ratios of 0.01%, 0.05%, 0.1%, 0.5%, 1%, 5%, and 100%. There are points for each position in light chain for each sample sequenced. The -axis corresponds to the apparent signal for each spiked-in sample. In order to include the negatives that result from this measurement on the log-scale plot, they are plotted as their absolute values, colored grey, and offset just below the other points. The points corresponding to the spiked-in mutations are colored blue and offset just above the other points. The light blue points did not meet the threshold for statistical significance. True-negative mutations that did meet the criteria for statistical significance are colored purple instead of black. All points have had a small amount of vertical jitter added. The jitter and offsets serve to allow visualization of the full distribution of points for the negative and positive controls.