Research Article
Quantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis
Table 1
Primer sequences for reference genes.
| | Gene symbol | Primer | NCBI accession numbers | PCR efficiency | Amplicon length |
| | ACTB | F: TGACGTTGACATCCGTAAAG | NM_007393.3 | 90.9% | 143 bp | | R: GAGGAGCAATGATCTTGATCT |
| | GAPDH | F: GGTGAAGGTCGGTGTGAACG | NM_001289726.1 | 101.9% | 233 bp | | R: CTCGCTCCTGGAAGATGGTG |
| | 18sRNA | F: GTAACCCGTTGAACCCCATT | NR_003278.3 | 95.1% | 151 bp | | R: CCATCCAATCGGTAGTAGCG |
| | B2M | F: GCTATCCAGAAAACCCCTCAA | NM_009735.3 | 96.2% | 300 bp | | R: CATGTCTCGATCCCAGTAGACGGT |
| | RPL13A | F: GAGGTCGGGTGGAAGTACCA | NM_009438.5 | 95.2% | 71 bp | | R: TGCATCTTGGCCTTTTCCTT |
| | EF1α | F: CTGAACCATCCAGGCCAAAT | NM_010106.2 | 98.0% | 60 bp | | R: GGCTGTGTGACAATCCAG |
| | UBC | F: GCCCAGTGTTACCACCAAGA | NM_019639.4 | 98.6% | 104 bp | | R: CCCATCACACCCAAGAACA |
| | HPRT1 | F: CCAGCGTCGTGATTAGCG | NM_013556.2 | 96.3% | 222 bp | | R: CCAGCAGGTCAGCAAAGAAC |
| | YWHAZ | F: CAGTAGATGGAGAAAGATTTGC | NM_001253807 | 97.4% | 92 bp | | R: GGGACAATTAGGGAAGTAAGT |
| | TUBB | F: CGGCAACTATGTAGGGGACT | NM_178014.3 | 96.7% | 194 bp | | R: CAGCACCACTCTGACCAAAG |
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