Research Article
Quantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis
Table 2
Comprehensive ranking of reference genes.
| | Ranking order | geNorm | NormFinder | BestKeeper | Variation of raw Ct values | Comprehensive ranking (mean rank value) |
| | 1 | RPL-13A
YWHAZ | YWHAZ | RPL-13A | RPL-13A | RPL-13A
YWHAZ
1.414 |
| | 2 | HPRT1 | GAPDH | YWHAZ | YWHAZ | HPRT1
3.080 |
| | 3 | GAPDH | UBC | HPRT1 | HPRT1 | GAPDH
4.242 |
| | 4 | EF-1α | RPL-13A | 18sRNA | EF-1α | UBC
4.786 |
| | 5 | UBC | HPRT1 | EF-1α | UBC | EF-1α
5.180 |
| | 6 | 18sRNA | B2M | GAPDH | 18sRNA | 18sRNA
6.160 |
| | 7 | B2M | TUBB | UBC | B2M | B2M
6.694 |
| | 8 | TUBB | ACTB | B2M | TUBB | TUBB
7.969 |
| | 9 | ACTB | EF-1α | TUBB | GAPDH | ACTB
9.212 |
| | 10 | — | 18sRNA | ACTB | ACTB | — |
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