Guitar: An R/Bioconductor Package for Gene Annotation Guided Transcriptomic Analysis of RNA-Related Genomic Features

Figure 1

Guitar coordinates. This figure illustrates how the Guitar coordinates are generated based on 3 bins on lncRNA transcript. The bins may be split into multiple sections of the transcript represented by GRangesList object, which can be conveniently compared with other genome-based features to reflect their distribution. In practice, if 70% of a feature overlap 5′UTR and 30% CDS, then it is likely that, the feature overlaps with more Guitar Coordinates (GCs) composed from 5′UTR than from CDS. As a result, more GCs from 5′UTR will report the overlapping of this feature, and the resulting weight will precisely reflect the 70% and 30% division.