Guitar: An R/Bioconductor Package for Gene Annotation Guided Transcriptomic Analysis of RNA-Related Genomic Features

Figure 3

m⁶A sites on mRNA and lncRNA. In mRNA, the strongest binding sites (“IP/input ratio” larger than 8) are highly enriched near stop codon side of 3′UTR and deficient on TSS (transcription starting site) side of 5′UTR and the phenomena are more prominent than lowly methylated sites. In contrast, the m⁶A sites are almost uniformly distributed on lncRNA despite the “IP/input ratio” specified. Please note that, in this figure, the size of 5′UTR, CDS, and 3′UTR reflects their true width within the transcriptome, so the 5′UTR region is much shorter compared with the other two components. This result is based on peaks called on human HepG2 dataset [10].