Azocasein Substrate for Determination of Proteolytic Activity: Reexamining a Traditional Method Using Bromelain Samples

Table 1

Rotational central composite design used to study and determine assay’s optimum conditions shown in Figure 1(c).

	Factor	Temperature (°C)	pH
Levels		37.93	6.17
		40.00	7.00
	0.000	45.00	9.00
	1.000	50.00	11.00
	1.414	52.07	11.80