Isolation and Characterization of a Broad Spectrum Bacteriocin from Bacillus amyloliquefaciens RX7

Table 1

Antimicrobial spectra of the RX7 bacteriocin.

Indicator organism	Media	Inhibition
		zone (mm)
Gram-positive
Bacillus cereus KCTC 1661	LB	++
Bacillus licheniformis KCCM 12145	NB	++
Enterococcus faecalis (VRE) CCARM 0011	NB	++
Listeria monocytogenes ATCC 19114	TSB	+++
Streptococcus agalactiae ATCC 13813	TSB	++
Staphylococcus aureus KFRI 00188	NB	++
Staphylococcus aureus subsp. aureus (MRSA) KCCM 40510	NB	+
Streptococcus mutans ATCC 25175	BHI	+
Lactobacillus acidophilus KCCM 32820	MRS	+
Lactobacillus delbrueckii KCCM 11357	MRS	+
Lactobacillus johnsonii PF01 (our isolate)	MRS	+
Lactobacillus salivarius CPM7 (our isolate)	MRS	+
Lactobacillus plantarum KCCM 11322	MRS	+
Gram-negative
Escherichia coli K88	LB	++
Klebsiella pneumoniae subsp. pneumoniae KCCM 11418	NB	+
Pseudomonas aeruginosa KCCM 11266	NB	++
Pseudomonas aeruginosa CCARM 2003	LB	++
Shigella flexneri KCCM 40414	NB	++
Salmonella enteritidis KCCM 12021	NB	++
Salmonella enteritidis KVCC-BA0700654	NB	++
Salmonella gallinarum KVCC-BA0700722	NB	++
Salmonella pullorum KVCC-BA0702509	NB	+
Salmonella typhimurium KCCM 40253	NB	++
Fungi
Candida albicans KCTC 7122	PDA	++

ATCC: American Type Culture Collection; CCARM: Culture Collection of Antimicrobial Resistant Microbes; KFRI: Kerala Forest Research Institute; KCCM: Korean Culture Center of Microorganisms; KCTC: Korean Collection for Type Culture.
TSB: tryptic soy broth; NB: nutrient broth; LB: Luria-bertani; BHI: brain heart infusion; MRS: de Man Rogosa and Sharpe.
Activity is expressed as the diameter of the inhibition zone around the well: +, less than 10 mm; ++, less than 20 mm; +++, less than 30 mm.