Isolation and Characterization of a Broad Spectrum Bacteriocin from Bacillus amyloliquefaciens RX7

Table 2

Effects of enzymes, temperature, pH, and organic solvents on the antimicrobial activity of RX7 bacteriocin.

Treatment	Residual activity (%)
None (control)	100
Enzymes^†
-Amylase	100
-Chymotrypsin	0
Lipase	100
Proteinase-K	0
Pepsin	100
Trypsin	0
Heat
4°C/30 min	100
37°C/30 min	100
50°C/30 min	100
80°C/30 min	100
100°C/30 min	80
121°C/15 min	20
pH
1	80
2	90
3	100
4	100
5	100
6	100
7	100
8	100
9	90
10	80
Organic solvents^‡
Acetone	90
Acetonitrile	90
Butanol	80
Chloroform	80
Ethanol	90
Methanol	80

Residual activity compared with antimicrobial activity before the treatment.
^†Enzyme concentrations were 10 mg/mL.
^‡10% concentration.