Research Article
A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA
Table 2
Sensitivity of RT-qPCR assays for VEEV, WEEV, and EEEV strains and determination of copy number.
| | RNA | Dilution | Ct | Copies/µL |
| | VEEV | 10−2 | 27,94 | 70200 | | 10−3 | 30,69 | 8840 | | 10−4 | 33,75 | 894 | | 10−5 | 35,51 | 240 | | 10−6 | 37,24 | 66 | | 10−7 | no Ct | 0 |
| | WEEV | 10−2 | 21,76 | 2880000 | | 10−3 | 25,13 | 216000 | | 10−4 | 28,64 | 13780 | | 10−5 | 31,1 | 2060 | | 10−6 | 32,38 | 736 | | 10−7 | no Ct | 0 |
| | EEEV | 10−3 | 29,67 | 12760 | | 10−4 | 32,55 | 1212 | | 10−5 | 34,22 | 316 | | 10−6 | no Ct | 0 | | 10−7 | no Ct | 0 |
|
|
