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BioMed Research International
Volume 2016, Article ID 8561251, 6 pages
http://dx.doi.org/10.1155/2016/8561251
Research Article

Clinical Significance of miR-149 in the Survival of Patients with Laryngeal Squamous Cell Carcinoma

1Department of Otorhinolaryngology, Tianjin Medical University General Hospital, Tianjin 300052, China
2Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China

Received 7 December 2015; Revised 5 February 2016; Accepted 24 May 2016

Academic Editor: Hushan Yang

Copyright © 2016 Yi Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

MicroRNAs (miRNAs) play critical roles in the progression of laryngeal cancer (LC). In this study, we aimed to investigate whether miR-149 is associated with the prognosis of patients with LC. A total of 97 laryngeal squamous cell carcinoma patients who underwent tumor resection were included in our follow-up study. In vitro studies was performed in cancer cell line Hep-2 to explore the antitumor role of miR-149 in LC. We found that the expression of miR-149 was significantly lower in tumor tissues, compared with vocal cord polyp tissues (). Kaplan-Meier analysis revealed that miR-149 expression status is significantly associated with survival duration (log rank test, ), and multivariate Cox regression analysis revealed that patients with low miR-149 expression had shorter survival times compared with patients with high miR-149 expression. In vitro studies revealed that the exogenous expression of miRNA-149 inhibits the proliferation of human Hep-2 cells and induces cell apoptosis. Our study suggests that miR-149 expression in laryngeal squamous cell carcinoma tissues is critically associated with the prognosis of patients, and the ectopic expression of miR-149 in Hep-2 cells inhibits proliferation and cell cycle progression.