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Figure 3: EF24 inhibits the migration and invasion of HCC. (a) EF24 treatment inhibits the migration of HCCLM-3 and HepG2 cells. The artificial wound was created by using a 200 μL pipette tip, and then a random field was chosen and photographed at 0 and 24 h, respectively. Representative images at 0 h and 24 h after wounding were shown at magnification of 100x. (b) Statistical analysis about the effect of EF24 on the migration of HCC. The wound width was measured and the healing ability was represented as a ratio of the 24 h width to 0 h width from the same field. (c) EF24 treatment inhibits the invasion of HCCLM-3 and HepG2 cells. After treatment with or without EF24 for 24 h, cell invasion ability was detected by transwell assay. The invaded cells were fixed, stained, and photographed under a light microscope. (d) Statistical analysis about the effect of EF24 on the invasion of HCC. Data are expressed as the average number of invaded cells under high power field from triplicate experiments. ; .