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Figure 5: EF24 inhibits the phosphorylation of Src in HCC cells. (a) Detection of the expression of t-Src and p-Src in HCCLM-3 and HepG2 cells treated with 1 μM EF24 by using Western blot assay. (b) Scanning densitometric analysis of Western blot visualizing the relative levels of t-Src and p-Src in HCCLM-3 and HepG2 cells treated with 1 μM EF24. EF24 treatment reduced p-Src but not t-Src level in HCCLM-3 cells. Data were shown as mean ± SD of three independent experiments, . (c) Detection of the expression of t-Src and p-Src in HCCLM-3 and HepG2 cells treated with 1 μM EF24 by using immunocytochemistry staining (×400). (d) Statistical analysis of the percentage of t-Src or p-Src staining positive cells. EF24 treatment reduced p-Src but not t-Src level in both HCCLM-3 and HepG2 cells. Data were shown as mean ± SD of three independent experiments, .