Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2016, Article ID 8580475, 9 pages
Research Article

Renal Ischemia/Reperfusion Injury in Diabetic Rats: The Role of Local Ischemic Preconditioning

1Department of Anesthesiology and Reanimation, School of Medicine, Dokuz Eylul University, Izmir, Turkey
2Department of General Surgery, School of Medicine, Dokuz Eylul University, Izmir, Turkey
3Department of Histology and Embryology, School of Medicine, Dokuz Eylul University, Izmir, Turkey
4Department of Experienced Laboratory Animal Science, School of Medicine, Dokuz Eylul University, Izmir, Turkey
5Department of Nutrition and Dietetics, Izmir Ataturk School of Health, Ege University, Izmir, Turkey

Received 3 November 2015; Revised 22 December 2015; Accepted 4 January 2016

Academic Editor: Jeremiah R. Brown

Copyright © 2016 Sule Ozbilgin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. The aim of this study was to evaluate the effects of local ischemic preconditioning using biochemical markers and histopathologically in the diabetic rat renal IR injury model. Methods. DM was induced using streptozotocin. Rats were divided into four groups: Group I, nondiabetic sham group (), Group II, diabetic sham group (), Group III, diabetic IR group (diabetic IR group, ), and Group IV, diabetic IR + local ischemic preconditioning group (diabetic IR + LIPC group, ). Ischemic renal injury was induced by clamping the bilateral renal artery for 45 min. 4 h following ischemia, clearance protocols were applied to assess biochemical markers and histopathologically in rat kidneys. Results. The histomorphologic total cell injury scores of the nondiabetic sham group were significantly lower than diabetic sham, diabetic IR, and diabetic IR + LIPC groups. Diabetic IR group scores were not significantly different than the diabetic sham group. But diabetic IR + LIPC group scores were significantly higher than the diabetic sham and diabetic IR groups. Conclusion. Local ischemic preconditioning does not reduce the risk of renal injury induced by ischemia/reperfusion in diabetic rat model.