Biochemical Characterization, Thermal Stability, and Partial Sequence of a Novel Exo-Polygalacturonase from the Thermophilic Fungus Rhizomucor pusillus A13.36 Obtained by Submerged Cultivation

Figure 4

Residual activity of the exo-PG from R. pusillus when incubated at different pH values for 24 hours at room temperature in the absence of substrate. Filled circles: crude extract; open circles: pure enzyme. The residual activity was calculated from a control sample that was prepared by mixing (1 : 1) the enzyme solution in water and was incubated at room temperature for 24 hours in the absence of the substrate (control = 100%). The average (symbols) and standard deviation (bars) are reported in the graph.