Biochemical Characterization, Thermal Stability, and Partial Sequence of a Novel Exo-Polygalacturonase from the Thermophilic Fungus Rhizomucor pusillus A13.36 Obtained by Submerged Cultivation

Table 1

Effect of cations (final concentration 10 mM) on the relative activity (%) of the exo-PG from R. pusillus partially purified. The asterisks designate significant differences between arithmetic means by Student’s -test () ± standard deviation. The experiments were performed at pH 5.0 at 60°C.

Ions (10 mM)	Mean (%)		SD (%)
Control		±	0.8
NaCl		±	8.0
MgCl2		±	3.5
AlCl3		±	7.8
KCl		±	0.7
FeCl3			—
CaCl2		±	0.8
BaCl2		±	6.5
MnCl2			—