Biochemical Characterization, Thermal Stability, and Partial Sequence of a Novel Exo-Polygalacturonase from the Thermophilic Fungus Rhizomucor pusillus A13.36 Obtained by Submerged Cultivation

Table 2

Purification table. The yield calculation considered as the initial condition the crude extract (100%). To calculate the purification factor, the initial condition (crude extract) with a purification factor 1 was considered. The salting-out condition refers to the supernatant obtained with 95% saturation of ammonium sulphate, and HIC stands for hydrophobic interaction chromatography.

Purification step	Volume (mL)	Total activity (U)	Total protein (mg)	Yield (%)	Specific activity (U/mg)	Purification factor
Crude extract	10.0	170.0	1.10	100.0	149.0	1.0
Salting-out	40.8	124.2	0.50	73.5	223.4	1.4
HIC	15.0	25.2	0.15	14.9	171.0	1.2