The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells

Figure 4

Effects of MB salt on OA-induced steatosis and TG accumulation. (a) HepG2 cells were left untreated or pretreated with CC for 30 min; then the cells were again left untreated or treated with OA or OA + MB salt as indicated. 24 h later, cells were used for ORO staining (upper panel) or harvested for intracellular TG assay (lower panel). Representative pictures are presented. Values are mean ± SD of 4 separate experiments; versus that of untreated cells; versus that of OA alone; versus that of OA + MB salt. (b) Cells were left untreated or treated with OA and an equal concentration of MB salt/M/B as indicated for 24 h. Then, cells were used for ORO staining (upper panel) or intracellular TG assay (lower panel). Representative pictures are presented. Values are mean ± SD of 4 separate experiments; versus that of untreated cells; and versus that of OA alone; versus that of OA + M or OA + B.

(a)

(b)