Ultrastructural Mapping of the Zebrafish Gastrointestinal System as a Basis for Experimental Drug Studies

Figure 1

Sample preparation and imaging workflow used for the observation and ultrastructural data correlation of a single zebrafish sample compatible with X-ray micro-CT, LM, and EM imaging modalities. LM imaging modality includes the array tomography technique, whereby serial sections are collected onto a glass slide and imaged using LM and back-scattered EM (BSEM). EM includes TEM, transmission electron tomography (TET), BSEM, and SBF-SEM. This sample preparation protocol not only allows for the sample to be compatible with all the different microscopy platforms but also provides superior ultrastructural preservation of the zebrafish larvae, compared to conventional protocols used for EM.