Ultrastructural Mapping of the Zebrafish Gastrointestinal System as a Basis for Experimental Drug Studies

Figure 4

Zebrafish larvae (12 dpf) model of liver (blue) and its vasculature (red), generated by serial LM imaging of 416 consecutive sections of 500 nm. (a) Dorsal view of the liver. (b) Vasculature of the liver (17% of total volume) represented in (a). (c) Combined liver model and its vasculature. (d) Same as (c), viewed from a different angle and clipped opened to visualise the internal vasculature. Liver vasculature was modelled by thresholding the grey values corresponding to the vessels and sinusoids from individual LM images. For full animation, see Supplementary information. Scale bar = 50 μm.

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