BioMed Research International
Volume 2016, Article ID 8758460, 13 pages
http://dx.doi.org/10.1155/2016/8758460
Ultrastructural Mapping of the Zebrafish Gastrointestinal System as a Basis for Experimental Drug Studies
1School of Medical Sciences (Discipline of Anatomy and Histology), The Bosch Institute, The University of Sydney, Sydney, NSW 2006, Australia
2Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW 2109, Australia
3Australian Centre for Microscopy & Microanalysis (ACMM), The University of Sydney, Sydney, NSW 2006, Australia
4Charles Perkins Centre, The University of Sydney, Sydney, NSW 2006, Australia
Received 17 March 2016; Accepted 4 May 2016
Academic Editor: Minjun Chen
Copyright © 2016 Delfine Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Supplementary Material
Supplementary Material include technical notes related to array tomography serial sections production and automated alignment using the ImageJ plugin StackReg. A comparative review of different sample preparation protocols and their effect on X-ray micro-CT imaging contrast are also presented. Finally, a 3D animation of a 12 dpf zebrafish liver and its vasculature as determined by thresholding methods from serial light microscopy sections.