BioMed Research International / 2016 / Article / Tab 2

Review Article

HDAC Inhibitors as Epigenetic Regulators of the Immune System: Impacts on Cancer Therapy and Inflammatory Diseases

Table 2

Assays to assess HDAC inhibitor effects.

Cell-based assaysReferences

Apoptosis [1, 33, 4244]
Cell cycle arrest[1, 3, 12, 45, 46]
Cytotoxicity[40, 41, 47]
Differentiation[2, 4]
Gene expression [1, 3, 33, 40]
Growth arrest[2, 3, 11]
Gene expression changes[1, 3, 33, 40]
Histone hyperacetylation[1, 3, 12, 39, 40, 47, 48]
Inflammation[4850]
Motility [1, 19, 26]
ROS induction[43, 51]
Senescence[5254]
Viability[1, 12, 40]

In vivo assaysReferences

Antiangiogenesis[5, 6]
Drug seeking behavior[55]
Oncogenesis[1, 40, 56, 57]
Senescence[58]
Thermogenesis[59]