Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo

Figure 4

Fluorescence imaging of PKH26⁺ cells in vitro and in vivo. (a) Duration of fluorescence after hUCMSC staining by PKH26 in vitro. (1) Nonpassage hUCMSCs marked with PKH26. (4) Four passages of hUCMSCs after PKH26 staining. ((2) and (5)) Cell nuclei stained with Hoechst33342 in the same field of vision as (1) and (4). (3, 6) Merged images. (b) Effect of PKH26 on hUCMSC growth curve. (c) Quantification of PKH26⁺ and EMR1⁺ cells in liver. (d) EMR1 immunofluorescence in frozen rat liver sections after transplantation of PKH26 marked hUCMSCs. Positive cells indicated by arrow (mean ± SD, , and compared with control). Bar = 100 μm. FOV, field of view.