Gamma-Ray Treatment of Echinococcus Protoscoleces prior to Implantation in Mice Reduces Echinococcosis

Figure 5

Calcium alizarin red staining of hydatid cysts. (a) Echinococcus granulosus stained uniformly and had no calcium deposition in the control group (×200). (b) (×50). (c) Echinococcus granulosus with 1/3 of the inner surface of the cuticle appearing to have calcium deposition in the 10 Gy group (×200). (d) (×400). (e) Whole Echinococcus granulosus cuticles had calcium salt deposits in the 20 Gy group (×50). (f) 20 Gy group hydatid cyst cuticle layer had calcium deposition, while inside the cuticle showed wave-like erosion (×400). (g) 40 Gy group Echinococcus granulosus cuticles had calcium salt deposits (×200). (h) 40 Gy group hydatid cyst layers had calcium deposition (×400). (i) 80 Gy group hydatid cyst cuticles collapsed and formed calcified lesions (×100). (j) Echinococcus cuticles collapsed and showed calcification in the 80 Gy group (×400). (k) Hydatid cyst cuticle fracture in the 80 Gy group (×50). (l) (×200). Gl: germinal layer; Cl: cuticle layer; and Ft: fibrous tissue.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)