Therapeutic Effects of CUR-Activated Human Umbilical Cord Mesenchymal Stem Cells on 1-Methyl-4-phenylpyridine-Induced Parkinson’s Disease Cell Model

Figure 4

Differentiation of PC12 PD model cells into neurons after treatment with CM-CUR, CM-MSC, and CUR. (a) The immunohistochemistry results showed the PD PC12 cells presented nuclear condensation, cell bodies sharply shrunk and rounded, and significantly decreased brown particles in the cytoplasm. However, after treatment with CM-CUR, CM-MSC, and CUR, the shrinkage of the cell bodies was significantly improved, the brown particles were significantly increased, and the cells appeared with different sizes and numbers (indicated with the arrows, bar is 10 μm). These phenomena were more obvious in the CM-CUR group. (b) The Western blot assay data showed that the MAP2 expression was gradually enhanced in the CM-CUR and CM-MSC groups and showed obvious significant differences compared with the model group (). ( the CM-CUR group versus the other two groups, CM-MSC and CUR groups versus the model group, CM-MSC groups versus CUR groups.)

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