Purification and Characterization of a New Thermostable, Haloalkaline, Solvent Stable, and Detergent Compatible Serine Protease from Geobacillus toebii Strain LBT 77

Table 1

Purification of protease from Geobacillus toebii LBT 77.

Purification step	Total activity (U)	Total protein (mg)	Specific activity (U/mg)	Recovery (%)	Purification fold
Crude enzyme	11357	171.5	66.5	100	1
Ammonium sulfate precipitation	8064	45	180	71	1.5
Sephadex G-75	2504	7	348	22	4.5
DEAE-Cellulose	2292.5	3	739.5	20	5