Mass Spectrometry-Based Metabolomic and Proteomic Strategies in Organic Acidemias

Figure 2

Schematic view of quantitative proteomic methods. Samples deriving from patients (i.e., tissues, cells, and body fluids) are the sources for clinical proteomics. Label-free and labeling proteomic approaches are the two main groups of MS-based strategies aimed at identifying and quantifying differentially expressed proteins between two different samples A and B (i.e., cells or tissues from OA patients versus healthy controls). Label-free methods include SpC and MS/MS TIC approaches. On the other hand, the other methods are based on metabolic labeling, such as SILAC, or chemical such as DIGE, ICAT, TMT, and iTRAQ.