Effects of Renal Ischemic Postconditioning on Myocardial Ultrastructural Organization and Myocardial Expression of Bcl-2/Bax in Rabbits

Figure 1

Comparison of cardiac histological changes and infarct area between experimental groups. The IR group underwent 60 min of left anterior descending coronary artery occlusion (LADO) and 6 h of reperfusion only. The RI-PostC group underwent 3 cycles of left renal artery occlusion for 30 seconds and release for 30 seconds before reperfusion. The RI-PostC + GF109203X group received 0.05 mg/kg GF109203X (protein kinase C inhibitor) intravenously for 10 min followed by RI-PostC. There were 12 rabbits in each group. (a) Light microscopy observations of HE-stained sections. IR group: granulocyte infiltration and a small quantity of red blood cell leakage were observed. RI-PostC group: mild myocardial interstitial edema was observed without granulocyte infiltration or red blood cell leakage. RI-PostC + GF109203X group: increased granulocyte numbers and leakage of red blood cells were observed. (b) Sections stained with NBT to identify regions of ischemia (arrows). (c) Comparison of infarct size between groups, calculated from experiments such as those shown in (b) ( per group). Infarct area was calculated as the percentage of the total cell area. compared with the IR group; compared with the RI-PostC + GF109203X group.

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