Effects of Renal Ischemic Postconditioning on Myocardial Ultrastructural Organization and Myocardial Expression of Bcl-2/Bax in Rabbits

Figure 3

Myocardial Bcl-2 and Bax protein expression levels. The IR group underwent 60 min of left anterior descending coronary artery occlusion (LADO) and 6 h of reperfusion only. The RI-PostC group underwent 3 cycles of left renal artery occlusion for 30 seconds and release for 30 seconds before reperfusion. The RI-PostC + GF109203X group received 0.05 mg/kg GF109203X (protein kinase C inhibitor) intravenously for 10 min followed by RI-PostC. There were 12 rabbits in each group. (a) Representative Western blot image showing the expression of Bcl-2, Bax, and β-actin in the 3 groups. (b) Expression of Bcl-2 relative to that of β-actin (). Bcl-2 protein expression was higher in the RI-PostC group than in the other 2 groups (). (c) Bax protein expression relative to that of β-actin (). Bax protein expression in the RI-PostC group was lower than that in the other 2 groups (). compared with the IR group; compared with the RI-PostC + GF109203X group.

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