Danggui Buxue Tang Attenuates Tubulointerstitial Fibrosis via Suppressing NLRP3 Inflammasome in a Rat Model of Unilateral Ureteral Obstruction

Figure 5

DBT inhibited the expression of NLRP3, ASC, pro-caspase-1, caspase-1, pro-IL-1β, and IL-1β in UUO rats by Western blot. UUO rats were given DBT and enalapril for treatment, and expressions of NLRP3, ASC, pro-caspase-1, caspase-1, pro-IL-1β, and IL-1β were measured by Western blot (a). Typical results are shown. In UUO group, NLRP3, ASC, pro-caspase-1, caspase-1, pro-IL-1β, and IL-1β were all significantly elevated. DBT and enalapril treatment decreased their expression (b, c, d, e). There were no differences between DBT and enalapril group, except for the expression of pro-IL-1β and IL-1β. DBT showed better significant effects on inhibiting the expression of pro-IL-1β and IL-1β compared to enalapril (e). All data were presented as means ± SD, ( versus sham group; versus UUO group; versus enalapril group).

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