Prenatal Dexamethasone Exposure Increases the Susceptibility to Autoimmunity in Offspring Rats by Epigenetic Programing of Glucocorticoid Receptor

Table 2

Primers for bisulfate-modified DNA.

Name	Primers	Length (bp)	CG sites	Region
Gene 1	F: 5′-aggaagagagTTAAGTGGTTTAAGGGTTTTTTATGA-3′	455	24	Promoter of exon 1-4
	R: 5′cagtaatacgactcactatagggagaaggctATAACCATAACTCCACCTCATNACC-3′
Gene 2	F: 5′-aggaagagagGGTNATGAGGTGGAGTTATGGTTAT-3′	395	18	Exon 1-4 and the promoter of exon 1-5
	R: 5′cagtaatacgactcactatagggagaaggctTTTCTAAATTCCAAAAACCCCTCTA-3′
Gene 3	F: 5′-aggaagagagGGGTTTTTGGAATTTAGAAAGTTGA-3′	461	27	Promoter of both exon 1-5 and exon 1-6
	R: 5′cagtaatacgactcactatagggagaaggctAATTACAAAACAAAACCCACCCT-3′
Gene 4	F: 5′-aggaagagagGGTGGGTTTTGTTTTGTAATTTTTT-3′	459	45	Promoter of both exon 1-6 and exon 1-7
	R: 5′cagtaatacgactcactatagggagaaggctATTTCTTTAANTTTCTCTTCTCCCAAA-3′
Gene 5	F: 5′-aggaagagagTTTTTTTATGGAGAAGAGGGGG-3′	428	50	Part of exon 1-10 and the promoter of exon 1-11
	R: 5′cagtaatacgactcactatagggagaaggctACAAAAACAAAACCAAAAAAAAC-3′
Gene 6	F: 5′-aggaagagagGTTTTTTTTGGTTTTGTTTTTGT-3′	448	26	Promoter of both exon 1-11 and exon 2
	R: 5′cagtaatacgactcactatagggagaaggctCCTCAAATATCAAAATAACACTTATCA-3′
Gene 7	F: 5′-aggaagagagTTGATTTTTTTGAGTGGTTTTTTTT-3′	498	18	Promoter of exon 2
	R: 5′cagtaatacgactcactatagggagaaggctAAATCCCCAATACAATAATAAACTTTTCC-3′