BioMed Research International / 2016 / Article / Tab 2

Research Article

Prenatal Dexamethasone Exposure Increases the Susceptibility to Autoimmunity in Offspring Rats by Epigenetic Programing of Glucocorticoid Receptor

Table 2

Primers for bisulfate-modified DNA.

NamePrimersLength (bp)CG sitesRegion

Gene 1F: 5′-aggaagagagTTAAGTGGTTTAAGGGTTTTTTATGA-3′45524Promoter of exon 1-4
R: 5′cagtaatacgactcactatagggagaaggctATAACCATAACTCCACCTCATNACC-3′
Gene 2F: 5′-aggaagagagGGTNATGAGGTGGAGTTATGGTTAT-3′39518Exon 1-4 and the promoter of exon 1-5
R: 5′cagtaatacgactcactatagggagaaggctTTTCTAAATTCCAAAAACCCCTCTA-3′
Gene 3F: 5′-aggaagagagGGGTTTTTGGAATTTAGAAAGTTGA-3′46127Promoter of both exon 1-5 and exon 1-6
R: 5′cagtaatacgactcactatagggagaaggctAATTACAAAACAAAACCCACCCT-3′
Gene 4F: 5′-aggaagagagGGTGGGTTTTGTTTTGTAATTTTTT-3′45945Promoter of both exon 1-6 and exon 1-7
R: 5′cagtaatacgactcactatagggagaaggctATTTCTTTAANTTTCTCTTCTCCCAAA-3′
Gene 5F: 5′-aggaagagagTTTTTTTATGGAGAAGAGGGGG-3′42850Part of exon 1-10 and the promoter of exon 1-11
R: 5′cagtaatacgactcactatagggagaaggctACAAAAACAAAACCAAAAAAAAC-3′
Gene 6F: 5′-aggaagagagGTTTTTTTTGGTTTTGTTTTTGT-3′44826Promoter of both exon 1-11 and exon 2
R: 5′cagtaatacgactcactatagggagaaggctCCTCAAATATCAAAATAACACTTATCA-3′
Gene 7F: 5′-aggaagagagTTGATTTTTTTGAGTGGTTTTTTTT-3′49818Promoter of exon 2
R: 5′cagtaatacgactcactatagggagaaggctAAATCCCCAATACAATAATAAACTTTTCC-3′

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